## ----include = FALSE---------------------------------------------------------- knitr::opts_chunk$set( collapse = TRUE, comment = "#>" ) ## ----include = FALSE---------------------------------------------------------- library(orthGS) ## ----eval=FALSE, include=TRUE------------------------------------------------- # # Plot species tree: # str <- ape::read.tree(text = "((((Pa,Psm),(Pp,Pin)),(Abi,Ap)),((Ara,(Pod,Nag)),(Sci,(Tba,Tax))));") # plot(str) # # Load GS sequence data: # agf <- agf # # Aligning sequences and building an unrooted tree # # (remember you need the MUSCLE software in your path): # conif <- agf[which(agf$short %in% str$tip.label), ] # ptr <- mltree(msa(sequences = conif$prot, # ids = conif$phylo_id, # inhouse = TRUE)$ali)$tree # # Rooting and plotting an ultrametric tree : # ptr <- madRoot(ptr) # plot(ptr, use.edge.length = FALSE, cex = 0.6) ## ----------------------------------------------------------------------------- data <- subsetGS(sp = c("Ap", "Abi", "Pin", "Pp", "Psm", "Pa"))[, 2:9] data$phylo_id ## ----eval=FALSE, include=TRUE------------------------------------------------- # o <- orthG(set = c("Ap", "Abi", "Pin", "Pp", "Psm", "Pa")) # A <- o[[1]] # Adjacency matrix # g <- o[[2]] # igraph object (orthology network) ## ----include=FALSE------------------------------------------------------------ g <- orthG(set = c("Ap", "Abi", "Pin", "Pp", "Psm", "Pa"))[[2]] ## ----eval = FALSE------------------------------------------------------------- # gs1a <- data$phylo[grepl("GS1a", data$phylo_id)] # selected nodes # plot(igraph::subgraph(g, vids = gs1a)) ## ----eval=FALSE, include=TRUE------------------------------------------------- # data <- sdf # gs1a <- data[which(data$gs == "GS1a" & data$tax_group != "Ferns"), ] ## ----eval = FALSE, include = TRUE--------------------------------------------- # o <- orthG() # g <- o[[2]] # plot(igraph::subgraph(g, vids = gs1a$Sec.Name_))